The TRAIL/ApoII Ligand (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant TRAIL/ApoIILigand concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0167
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
TRAIL/ApoIILigand
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P50591
NCBI Gene ID
8743
Target Synonyms
Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253
Human TRAIL/ApoIILigand recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human TRAIL/ApoIILigand was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for TRAIL/ApoIILigand. Each known sample concentration was assayed in triplicate. The OmniKine™ Human TRAIL/ApoII Ligand ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TRAIL/ApoII Ligand proteins within the range of 32-2000 pg/ml.
