The TWEAK (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant TWEAK concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0169
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
TWEAK
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
O43508
NCBI Gene ID
8742
Target Synonyms
Tumor necrosis factor ligand superfamily member 12, APO3 ligand, TNF-related weak inducer of apoptosis, APO3L, DR3LG
Human TWEAK recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human TWEAK was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for TWEAK. Each known sample concentration was assayed in triplicate. The OmniKine™ Human TWEAK ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TWEAK proteins within the range of 16-2000 pg/ml.
Author:
Clara Domınguez, MD ; Alba Vieites-Prado, BsC; Marıa P erez-Mato, PhD; Tom as Sobrino, PhD;
Xiana Rodrıguez-Osorio, MD, PhD; Ana Lopez, MD, PhD; Francisco Campos, PhD;
Francisco Martınez, MD, PhD; Jos e Castillo, MD, PhD; Rogelio Leira, MD, PhD