The IL-1 R1 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant IL-1R1 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0228
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
IL-1R1
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P14778
NCBI Gene ID
3554
Target Synonyms
Interleukin-1 receptor type 1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, p80, IL1R, IL1RA, IL1RT1
Human IL-1R1 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human IL-1R1 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for IL-1R1. Each known sample concentration was assayed in triplicate. The OmniKine™ Human IL-1 R1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-1 R1 proteins within the range of 125-8000 pg/ml.