The IL-13 Ralpha1 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant IL-13Ralpha1 concentrations within any experimental sample including cell lysates, serum and plasma.
Human IL-13Ralpha1 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human IL-13Ralpha1 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for IL-13Ralpha1. Each known sample concentration was assayed in triplicate. The OmniKine™ Human IL-13 Ralpha1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-13 Ralpha1 proteins within the range of 250-16000 pg/ml.
