The MIP-3alpha (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant MIP-3alpha concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0255
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
MIP-3alpha
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P78556
NCBI Gene ID
6364
Target Synonyms
C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20
Human MIP-3alpha recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human MIP-3alpha was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for MIP-3alpha. Each known sample concentration was assayed in triplicate. The OmniKine™ Human MIP-3alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MIP-3alpha proteins within the range of 16-1000 pg/ml.