The BCA-1 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant BCA-1 concentrations within any experimental sample including cell lysates, serum and plasma.
Human BCA-1 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human BCA-1 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for BCA-1. Each known sample concentration was assayed in triplicate. The OmniKine™ Human BCA-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human BCA-1 proteins within the range of 16-1000 pg/ml.
