The Osteopontin (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Osteopontin concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0344
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
Osteopontin
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P10451
NCBI Gene ID
6696
Target Synonyms
Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin
Human Osteopontin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Osteopontin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Osteopontin. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Osteopontin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Osteopontin proteins within the range of 63-4000 pg/ml.