The sTNF-R2 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant sTNF-R2 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0355
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
sTNF-R2
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
P20333
NCBI Gene ID
7133
Target Synonyms
Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, INN=Etanercept, TNFBR, TNFR2
Human sTNF-R2 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human sTNF-R2 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for sTNF-R2. Each known sample concentration was assayed in triplicate. The OmniKine™ Human sTNF-R2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TNF-R2 proteins within the range of 8-500 pg/ml.
