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Histone H3 (Acetyl-Lys27) Antibody
Histone H3 (Acetyl-Lys27) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: D0010
Reactivity:Human,Mouse,Rat,Monkey,Arabidopsis thaliana
Applications:WB,IHC,IF,IP,ELISA
Modification:Acetyl Specific
Source:Store at -20°C/1 year
Storage and Stability:

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Histone H3 (Acetyl-Lys27) Antibody

Immunofluorescence analysis of Hela cell. 1,Histone H3 (Acetyl Lys27) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(4B10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).

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Product Details
Product Name
Histone H3 (Acetyl-Lys27) Antibody
Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
Catalog No
D0010
Reactivity
Human, Mouse, Rat, Monkey, Arabidopsis thaliana
Source
Polyclonal, Rabbit,IgG
Modification
Acetyl Specific
Applications
WB, IHC, IF, IP, ELISA
Dilution
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. IP 1:200 ELISA: 1:5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3; H3k27AC
Molecular Weight (Da or kDa)
Gene Name
HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B
Protein Name
Histone H3.1/Histone H3.2/Histone H3.3
Human Gene ID
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/126961/333932/653604/3020/3021
Human Swiss Prot No.
P68431 / Q71DI3 / P84243
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys27. AA range:11-60
Specificity
Acetyl-Histone H3 (K27) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K27.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (3)
Comprehensive landscape of epigenetic-dysregulated lncRNAs reveals a profound role of enhancers in carcinogenesis in breast cancer subtypes. Molecular Therapy-Nucleic Acids 2021 Jan 01 WB Human 1:500 MDA-MB-468s

Author: Available in link provided

Date of Publication : Available in link provided

Up-regulation of neprilysin mediates the protection of fructo-oligosaccharides against Alzheimer's disease. Food & Function Food Funct. 2020 Jul;11(7):6565-6572 WB Mouse,Human 1:200 Cerebral cortex SH-SY5Ycell

Author: Available in link provided

Date of Publication : Available in link provided

AtHD2D, A Plant-specific Histone Deacetylase Involved in Abscisic Acid Response and Lateral Root Development JOURNAL OF EXPERIMENTAL BOTANY Zhaofen Han WB Arabidopsis thaliana

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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