Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
Catalog No
D0012
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys8) Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of KB cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Western Blot analysis of various cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys8) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).
