This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations invo
Catalog No
D0019
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CREBBP; CBP; CREB-binding protein
Molecular Weight (Da)
265351
Gene Name
CREBBP
Protein Name
CREB-binding protein
Human Gene ID
1387
Human Swiss Prot No.
Q92793
Immunogen
The antiserum was produced against synthesized peptide derived from human CBP around the acetylated site of Lys1535. AA range:1501-1550
Specificity
Acetyl-CBP (K1535) Polyclonal Antibody detects endogenous levels of CBP protein only when acetylated at K1535.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CBP (Acetyl-Lys1535) Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using Acetyl-CBP (K1535) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
