The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
Catalog No
D12057
Reactivity
Human:K117, Mouse:K112, Rat:K115
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
wb dilution 1:2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
Transcription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1)
Molecular Weight (Da)
Gene Name
E2F1 RBBP3
Protein Name
E2F-1
Human Gene ID
1869
Human Swiss Prot No.
Q01094
Immunogen
Synthesized Acetyl peptide derived from human E2F-1. at AA range: K117
Specificity
This antibody detects endogenous levels of E2F-1 (Acetyl-K117), It doesn't reacte with total protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of 1,hela 2,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
