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> Products > Primary Antibodies > Cleaved-Notch 2 (D1733) Antibody

Cleaved-Notch 2 (D1733) Antibody

Overview Description
Datasheet SDS
   Membrane Receptors   
Catalog No : L0120
  • Reactivity
    Human,Mouse,Rat
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Cleaved Specific
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    Cleaved-Notch 2 (D1733) Antibody
  • Description
    notch 2(NOTCH2) Homo sapiens This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cle
  • Catalog No
    L0120
  • Reactivity
    Human, Mouse, Rat
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Cleaved Specific
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2
  • Molecular Weight (Da)
    265404
  • Gene Name
    NOTCH2
  • Protein Name
    Neurogenic locus notch homolog protein 2
  • Human Gene ID
    4853
  • Human Swiss Prot No.
    Q04721
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Notch 2. AA range:1684-1733
  • Specificity
    Cleaved-Notch 2 (D1733) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to D1733.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from 293 cells, treated with TNF-a 20ng/ml 30', using Notch 2 (Cleaved-Asp1733) Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Notch 2 (Cleaved-Asp1733) Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
The picture was kindly provided by our customer,antibody was diluted at 1:500
Application Data
Western Blot analysis of 293 cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of various cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody diluted at 1:500
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Hela cell. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Application Data
Ylivinkka, Irene, et al. "Motility of glioblastoma cells is driven by netrin-1 induced gain of stemness." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 9.
Application Data
Pu, Y., Song, Y., Zhang, M. et al. GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. Sig Transduct Target Ther 6, 148 (2021).
Publications

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