notch 4(NOTCH4) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which
Catalog No
L0363
Reactivity
Human, Mouse, Monkey
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NOTCH4; INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4
Molecular Weight (Da)
209622
Gene Name
NOTCH4
Protein Name
Neurogenic locus notch homolog protein 4
Human Gene ID
4855
Human Swiss Prot No.
Q99466
Immunogen
The antiserum was produced against synthesized peptide derived from human NOTCH4. AA range:1401-1450
Specificity
Cleaved-Notch 4 (V1432) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 4 protein resulting from cleavage adjacent to V1432.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 and COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is blocked with the synthesized peptide.
