The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the Cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]
Catalog No
A0413
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6
Molecular Weight (Da)
62720
Gene Name
CDC6
Protein Name
Cell division control protein 6 homolog
Human Gene ID
990
Human Swiss Prot No.
Q99741
Immunogen
The antiserum was produced against synthesized peptide derived from human CDC6 around the phosphorylation site of Ser54. AA range:20-69
Specificity
Phospho-Cdc6 (S54) Polyclonal Antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using CDC6 (Phospho-Ser54) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CDC6 (Phospho-Ser54) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC6 (Phospho-Ser54) Antibody
