This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the Cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the Cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript varian
Catalog No
A0419
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DAXX; BING2; DAP6; Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein
Molecular Weight (Da)
81373
Gene Name
DAXX
Protein Name
Death domain-associated protein 6
Human Gene ID
1616
Human Swiss Prot No.
Q9UER7
Immunogen
The antiserum was produced against synthesized peptide derived from human Daxx around the phosphorylation site of Ser668. AA range:634-683
Specificity
Phospho-Daxx (S668) Polyclonal Antibody detects endogenous levels of Daxx protein only when phosphorylated at S668.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with PBS 60', using Daxx (Phospho-Ser668) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human ovary, using Daxx (Phospho-Ser668) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Daxx (Phospho-Ser668) Antibody
