The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
Catalog No
A0425
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
FADD; MORT1; GIG3; Protein FADD; FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity
Molecular Weight (Da)
23279
Gene Name
FADD
Protein Name
Protein FADD
Human Gene ID
8772
Human Swiss Prot No.
Q13158
Immunogen
The antiserum was produced against synthesized peptide derived from human FADD around the phosphorylation site of Ser194. AA range:159-208
Specificity
Phospho-FADD (S194) Polyclonal Antibody detects endogenous levels of FADD protein only when phosphorylated at S194.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60', using FADD (Phospho-Ser194) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FADD (Phospho-Ser194) Antibody. The picture on the right is blocked with the phospho peptide.