This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Catalog No
A0494
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
INSR; Insulin receptor; IR; CD antigen CD220
Molecular Weight (Da)
156307
Gene Name
INSR
Protein Name
Insulin receptor
Human Gene ID
3643
Human Swiss Prot No.
P06213
Immunogen
The antiserum was produced against synthesized peptide derived from human IR around the phosphorylation site of Tyr1361. AA range:1331-1380
Specificity
Phospho-Insulin R (Y1361) Polyclonal Antibody detects endogenous levels of Insulin R protein only when phosphorylated at Y1361.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with Heat shock, using IR (Phospho-Tyr1361) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IR (Phospho-Tyr1361) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IR (Phospho-Tyr1361) Antibody
Western blot analysis of Insulin R (phospho Tyr1361) Polyclonal Antibody, using hela,293 cell treated or untreated with EGF 200ng/ml 30', 4° over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37° 1hour.