The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Catalog No
A0635
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Molecular Weight (Da)
48337
Gene Name
CCNB1
Protein Name
G2/mitotic-specific cyclin-B1
Human Gene ID
891
Human Swiss Prot No.
P14635
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser147. AA range:121-170
Specificity
Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with UV 15', using Cyclin B1 (Phospho-Ser147) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human placenta, using Cyclin B1 (Phospho-Ser147) Antibody. The picture on the right is blocked with the phospho peptide.