The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in
Catalog No
A0636
Reactivity
Human, Rat, Mouse
Applications
IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CCNE1; CCNE; G1/S-specific cyclin-E1
Molecular Weight (Da)
47077
Gene Name
CCNE1
Protein Name
G1/S-specific cyclin-E1
Human Gene ID
898
Human Swiss Prot No.
P24864
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77. AA range:43-92
Specificity
Phospho-Cyclin E1 (T77) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T77.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
