This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
Catalog No
A0818
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
APC; DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
Molecular Weight (Da)
311646
Gene Name
APC
Protein Name
Adenomatous polyposis coli protein
Human Gene ID
324
Human Swiss Prot No.
P25054
Immunogen
The antiserum was produced against synthesized peptide derived from human APC around the phosphorylation site of Ser2054. AA range:2020-2069
Specificity
Phospho-APC (S2054) Polyclonal Antibody detects endogenous levels of APC protein only when phosphorylated at S2054.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using APC (Phospho-Ser2054) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using APC (Phospho-Ser2054) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using APC (Phospho-Ser2054) Antibody
