Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by Ref
Catalog No
A1038
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HRH1; Histamine H1 receptor; H1R; HH1R
Molecular Weight (Da)
55784
Gene Name
HRH1
Protein Name
Histamine H1 receptor
Human Gene ID
3269
Human Swiss Prot No.
P35367
Immunogen
The antiserum was produced against synthesized peptide derived from human Histamine H1 Receptor around the phosphorylation site of Ser398. AA range:364-413
Specificity
Phospho-Histamine H1 Receptor (S398) Polyclonal Antibody detects endogenous levels of Histamine H1 Receptor protein only when phosphorylated at S398.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunofluorescence analysis of HUVEC cells, using Histamine H1 Receptor (Phospho-Ser398) Antibody. The picture on the right is blocked with the phospho peptide.
