This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Catalog No
A7113
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54
Molecular Weight (Da)
57825
Gene Name
ICAM1
Protein Name
Intercellular adhesion molecule 1
Human Gene ID
3383
Human Swiss Prot No.
P05362
Immunogen
The antiserum was produced against synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyr512. AA range:479-528
Specificity
Phospho-ICAM-1 (Y512) Polyclonal Antibody detects endogenous levels of ICAM-1 protein only when phosphorylated at Y512.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with TNF-alpha 20ng/ml 5', using ICAM-1 (Phospho-Tyr512) Antibody. The lane on the right is blocked with the phospho peptide.