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Modification-Specific Antibodies » Myc (Phospho-Ser62) Antibody

Catalog No Size Price  
A0020 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 48 kDa Rabbit
Note:

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Product Name Myc (Phospho-Ser62) Antibody
Synonyms Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64 ; MYC ; BHLHE39
Immunogen The antiserum was produced against synthesized peptide derived from human Myc around the phosphorylation site of Ser62.
Immunogen Range 31-80
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity Myc (Phospho-Ser62) Antibody detects endogenous levels of Myc only when phosphorylated at Ser62.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol MYC
Database Links

Gene ID: 9531 Swiss-Prot #: P01106

OMIM #: 190080 Unigene #: Hs.202453

Target Modification Phospho
Modification Sites H:S62 M:S62 R:S62
Application Images
Image 1 Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from 293 cells treated with Forskolin 40nM 30', using Myc (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.