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Modification-Specific Antibodies » Keratin 8 (Phospho-Ser73) Antibody

Catalog No Size Price  
A0017 $175
Reactivity Applications MW Source
H, M WB IHC ELISA 53 kDa Rabbit
Note:

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Product Name Keratin 8 (Phospho-Ser73) Antibody
Synonyms CK 8; CK8; CYK8; Cytokeratin endo A; K2C8; K8; KRT2-8; KRT8; Keratin, type II cytoskeletal 8; cytokeratin 8
Immunogen The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser73.
Immunogen Range 41-90
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity Keratin 8 (Phospho-Ser73) Antibody detects endogenous levels of Keratin 8 only when phosphorylated at Ser73.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol KRT8
Database Links

Gene ID: 3856 Swiss-Prot #: P05787

OMIM #: 148060/215600 Unigene #: Hs.533782/Hs.708445

Target Modification Phospho
Modification Sites H:S73 M:S79
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 8 (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Keratin 8 (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.