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Modification-Specific Antibodies » Keratin 18 (Phospho-Ser33) Antibody

Catalog No Size Price  
A0016 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 47 kDa Rabbit
Note:

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Product Name Keratin 18 (Phospho-Ser33) Antibody
Synonyms CK 18; CK18; CYK18; Cytokeratin endo B; K18; K1C18; KERD; KRT1-18; KRT18; Keratin; Keratin D; Keratin; type I cytoskeletal 18; cytokeratin 18; type I cytoskeletal 18
Immunogen The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33.
Immunogen Range 1-50
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity Keratin 18 (Phospho-Ser33) Antibody detects endogenous levels of Keratin 18 only when phosphorylated at Ser33.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol KRT18
Database Links

Gene ID: 3875 Swiss-Prot #: P05783

OMIM #: 148070/215600 Unigene #: Hs.406013

Target Modification Phospho
Modification Sites H:S34 M:S35 R:S35
Application Images
Image 1 Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HT29 cells, using Keratin 18 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.