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Modification-Specific Antibodies » IkappaB-beta (Phospho-Ser23) Antibody

Catalog No Size Price  
A0014 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 37 kDa Rabbit
Note:

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Product Name IkappaB-beta (Phospho-Ser23) Antibody
Synonyms I-kappa-B-beta; IKB-B; IKBB; IkappaBbeta; NF-kappa-BIB; NF-kappaB inhibitor beta; NFKBIB, TR- interacting protein 9; TRIP9; Thyroid receptor interacting protein 9
Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23.
Immunogen Range 8-57
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity IkappaB-beta (Phospho-Ser23) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at Ser23.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol NFKBIB
Database Links

Gene ID: 4793 Swiss-Prot #: Q15653

OMIM #: 604495 Unigene #: Hs.9731

Target Modification Phospho
Modification Sites H:S23 M:S23 R:S23
Application Images
Image 1 Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.