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Modification-Specific Antibodies » HSP90B (Phospho-Ser254) Antibody

Catalog No Size Price  
A0013 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 83 kDa Rabbit
Note:

The product is for research use only, not for use in diagnostic or therapeutic procedures

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Product Name HSP90B (Phospho-Ser254) Antibody
Synonyms HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; HSPCB; Heat shock protein HSP 90-beta
Immunogen The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254.
Immunogen Range 226-275
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at Ser254.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol HSP90AB1
Database Links

Gene ID: 3326 Swiss-Prot #: P08238

OMIM #: 140572 Unigene #: Hs.509736/Hs.689683

Target Modification Phospho
Modification Sites H:S254 M:S254 R:S254
Application Images
Image 1 Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is blocked with the phospho peptide.