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Modification-Specific Antibodies » GSK3 alpha/beta (Phospho-Tyr279/216) Antibody

Catalog No Size Price  
A0012 $175
Reactivity Applications MW Source
H, M, R WB IHC ELISA 50 kDa Rabbit
Note:

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Product Name GSK3 alpha/beta (Phospho-Tyr279/216) Antibody
Synonyms Factor A; GSK-3 alpha; GSK3-alpha; Glycogen synthase kinase-3 alpha; kinase GSK3-alpha
Immunogen The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216.
Immunogen Range 246-295
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity GSK3 alpha/beta (Phospho-Tyr279/216) Antibody detects endogenous levels of GSK3 alpha/beta only when phosphorylated at Tyr279/216.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol GSK3A
Database Links

Gene ID: 2931 Swiss-Prot #: P49840

OMIM #: 606784 Unigene #: Hs.466828

Target Modification Phospho
Modification Sites H:Y279 M:Y279 R:Y279
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from HeLa cells, using GSK3 alpha/beta (Phospho-Tyr279/216) Antibody. The lane on the right is blocked with the phospho peptide.