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Modification-Specific Antibodies » EGFR (Phospho-Thr693) Antibody

Catalog No Size Price  
A0009 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 134 kDa Rabbit
Note:

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Product Name EGFR (Phospho-Thr693) Antibody
Synonyms Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 ; EGFR ; ERBB1
Immunogen The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr693.
Immunogen Range 661-710
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity EGFR (Phospho-Thr693) Antibody detects endogenous levels of EGFR only when phosphorylated at Thr693.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol EGFR
Database Links

Gene ID: 1956 Swiss-Prot #: P00533

OMIM #: 131550/211980 Unigene #: Hs.488293

Target Modification Phospho
Modification Sites H:T693 M:T695 R:T694
Application Images
Image 1 Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr693) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Thr693) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from A431 cells, using EGFR (Phospho-Thr693) Antibody. The lane on the right is blocked with the phospho peptide.