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Modification-Specific Antibodies » EGFR (Phospho-Thr678) Antibody

Catalog No Size Price  
A0008 $175
Reactivity Applications MW Source
H, M, R WB IF ELISA 134 kDa Rabbit
Note:

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Product Name EGFR (Phospho-Thr678) Antibody
Synonyms Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 ; EGFR ; ERBB1
Immunogen The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678.
Immunogen Range 651-700
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Specificity EGFR (Phospho-Thr678) Antibody detects endogenous levels of EGFR only when phosphorylated at Thr678.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol EGFR
Database Links

Gene ID: 1956 Swiss-Prot #: P00533

OMIM #: 131550/211980 Unigene #: Hs.488293

Target Modification Phospho
Modification Sites H:T678 M:T680 R:T679
Application Images
Image 1 Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using EGFR (Phospho-Thr678) Antibody. The lane on the right is blocked with the phospho peptide.