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Modification-Specific Antibodies » DARPP-32 (Phospho-Thr75) Antibody

Catalog No Size Price  
A0007 $175
Reactivity Applications MW Source
H, M, R WB IHC ELISA 22 kDa Rabbit
Note:

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Product Name DARPP-32 (Phospho-Thr75) Antibody
Synonyms Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B
Immunogen The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75.
Immunogen Range 41-90
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr75.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol PPP1R1B
Database Links

Gene ID: 84152 Swiss-Prot #: Q9UD71

OMIM #: 604399 Unigene #: Hs.286192

Target Modification Phospho
Modification Sites H:T75 M:T75 R:T75
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is blocked with the phospho peptide.