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Modification-Specific Antibodies » CREB (Phospho-Ser142) Antibody

Catalog No Size Price  
A0006 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 36 kDa Rabbit
Note:

The product is for research use only, not for use in diagnostic or therapeutic procedures

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Product Name CREB (Phospho-Ser142) Antibody
Synonyms cAMP responsive element binding protein 1; cAMP-response element binding protein; CREB-1; CREB1
Immunogen The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser142.
Immunogen Range 111-160
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity CREB (Phospho-Ser142) Antibody detects endogenous levels of CREB only when phosphorylated at Ser142.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol CREB1
Database Links

Gene ID: 1385 Swiss-Prot #: P16220

OMIM #: 123810/612160 Unigene #: Hs.516646/Hs.584750

Target Modification Phospho
Modification Sites H:S142 M:S142 R:S142
Application Images
Image 1 Immunofluorescence analysis of HeLa cells, using CREB (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CREB (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using CREB (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.