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Modification-Specific Antibodies » AMPK alpha 1/2 (Phospho-Thr183/172) Antibody

Catalog No Size Price  
A0003 $175
Reactivity Applications MW Source
H, M, R WB IHC ELISA 62 kDa Rabbit
Note:

The product is for research use only, not for use in diagnostic or therapeutic procedures

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Product Name AMPK alpha 1/2 (Phospho-Thr183/172) Antibody
Synonyms

5'-AMP-activated protein kinase; catalytic alpha-1 chain; AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1; acetyl-CoA carboxylase kinase; hormone-sensitive lipase kinase; kinase AMPK-alpha1; 5'-AMP-activated protein kinase catalyt; PRKAA2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; AMPK, AMPK2; PRKAA2

Immunogen The antiserum was produced against synthesized peptide derived from human AMPK alpha 1/2 around the phosphorylation site of Thr183/172.
Immunogen Range 140-189
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity AMPK alpha 1/2 (Phospho-Thr183/172) Antibody detects endogenous levels of AMPK alpha 1/2 only when phosphorylated at Thr183/172.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol PRKAA1; PRKAA2
Database Links

Gene ID: 5562/5563 Swiss-Prot #: Q13131/P54646

OMIM #: 602739/600497 Unigene #: Hs.43322/Hs.437039

Target Modification Phospho
Modification Sites H:T183/172 M:T183/172 R:T183/172
Application Images
Image 1 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha 1/2 (Phospho-Thr183/172) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from COS7 cells, using AMPK alpha 1/2 (Phospho-Thr183/172) Antibody. The lane on the right is blocked with the phospho peptide.