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Modification-Specific Antibodies » ADD1 (Phospho-Ser726) Antibody

Catalog No Size Price  
A0002 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 80 kDa Rabbit
Note:

The product is for research use only, not for use in diagnostic or therapeutic procedures

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Product Name ADD1 (Phospho-Ser726) Antibody
Synonyms Alpha-adducin; Erythrocyte adducin subunit alpha ; ADD1 ; ADDA
Immunogen The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726.
Immunogen Range 688-737
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity ADD1 (Phospho-Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Ser726.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol ADD1
Database Links

Gene ID: 118/119 Swiss-Prot #: P35611/P35612

OMIM #: 102680/102681 Unigene #: Hs.183706/Hs.701490/Hs.188528

Target Modification Phospho
Modification Sites H:S726/S713 M:S724/S712 R:S724/S712
Application Images
Image 1 Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. The lane on the right is blocked with the phospho peptide.