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Modification-Specific Antibodies » 14-3-3 zeta (Phospho-Ser58) Antibody

Catalog No Size Price  
A0001 $175
Reactivity Applications MW Source
H, M, R WB IHC IF ELISA 27 kDa Rabbit
Note:

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Product Name 14-3-3 zeta (Phospho-Ser58) Antibody
Synonyms

14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ

Immunogen The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58.
Immunogen Range 24-73
Clonality Polyclonal
Application Dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity 14-3-3 zeta (Phospho-Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability Stable at -20°C for at least 1 year.
NCBI Gene Symbol YWHAZ
Database Links

Gene ID: 7534 Swiss-Prot #: P63104

OMIM #: 601288 Unigene #: Hs.492407/Hs.594673

Target Modification Phospho
Modification Sites H:S58 M:S58 R:S58
Application Images
Image 1 Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Image 3 Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.