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Polyclonal Antibodies

Assay Biotech has been a primary manufacturer of high quality rabbit and mouse antibodies since 2006. With over 10,000 antibodies on the market today. We specialize in total protein and modification-specific antibodies for phosphorylated, methylated, and acetylated proteins in a multitude of pathways.

Unmodified and total protein antibodies are capable of detecting unmodified proteins as well as total amounts of the target protein that have undergone various types of post-translational modification, respectively.

Modification-specific antibodies are capable of detecting proteins post-translationally modified via acetylation. methylation, cleavage, mutation and phosphorylation at certain amino acids along the target protein.

Assay Biotech antibodies are validated via Western Blot (WB), Enzyme Linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), and Immunofuorescence (IF).

Over 10,000 highly specific primary antibodies.

High sensitivity, low background with exceptional signal to noise ratio.

Each antibody is produced in rabbits using syntheic immunogen peptides and purfied via immungen specific affinity chromatography.

Monoclonal Antibodies

Monoclonal antibodies are antibodies that are derived from the same clonal population of immune cells. This means that monoclonal antibodies are identical at the DNA sequence level, meaning that they all recognise the same epitope.

Monoclonal antibodies have many benefits in any area that uses antibodies, such as western blotting, immunohistochemistry and immunofluorescence. Since these antibodies recognise the same epitope, they are less likely to non-specifically cross-react with non-target proteins, thus increasing overall signal-noise ratios in WB, IHC and IF; producing much clearer images.

The specificity of monoclonal antibodies significantly reduces the amount of background noise in IHC data, making both identification and quantification easier, which can improve consistency in analysis between experimenters.

Exceptional specificity and reduction of background for ease of identification and quanitification.

Low staining artifiacts.

Antibodies heavily validated for Immunohistochemistry application.

Highly consistent results across diferent batches.

Secondary Antibodies

Secondary antibodies are antibodies that recognise and bind the constant region of other antibodies. These are used in any application that uses indirect methods of detection for proteins such as Western blot, ELISA, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry. Secondary antibodies are designed to recognise the constant regions of specific species of antibodies, thus allowing multiplexing and detection of many different target proteins in tandem.

Typically, secondary antibodies are pre-adsorbed to prevent cross-reactivity with antibodies of other species. This is often done by passing the secondary antibody solution through a column that contains immunoglobulins from other, non-target species. Secondary antibodies that cross-react with other species of antibodies are unable to elute and are thus removed from the final solution. Most distributors and manufacturers employ this quality-control, which helps prevent non-specific binding in experiments and thus improves reproducibility of results.

Antibodies availble for detection in IHC, WB, IF, IP, ELISA, and Flow Cytometry.

Conjugated fluorescent dyes available in a wide spectrum.

In-direct binding leading to amplification of signal to noise ratio.

Pre=adsorbed antibodies to prevent cross reactivity.