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Cell-Based Phospho ELISA Kits

Cell-Based Phospho ELISA kits

The CytoGlow™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Features

  • Detection of phosphorylated protein markers in adherent and suspension cells
  • Convenient and ready-to-use
  • Sample preparation not necessary
  • Minimum of 5,000 cells required per well
  • High-sensitivity, low background with exceptional signal-to-noise ratio
  • 450 nm colorimetric detection
  • Can be used with human mouse and rat targets in their respective cell lines

Kit Components

  • 2 x 96-Well Tissue Culture Microplates
  • 100x Anti-Phospho Target Primary Antibody
  • 100x Anti-Target Primary Antibody
  • 100x Anti-Control Primary Antibody
  • HRP-Conjugated Anti-Rabbit IgG Secondary Antibody
  • HRP-Conjugated Anti-Mouse IgG Secondary Antibody
  • 10x TBS
  • 10x Wash Buffer
  • Quenching Buffer
  • Blocking Buffer
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
  • Technical Manual