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Antibodies

Assay Biotech is a primary manufacturer of over 10,000 highly specific primary antibodies against targets implicated in various cellular processes including but not limited to cell adhesion, cancer, cell cycle, apoptosis, vesicle trafficking, signal transduction and macromolecular biosynthesis. Each antibody is produced in rabbits using synthetic immunogen peptides and purified via immunogen-specific affinity chromatography.

Modification-Specific Antibodies

Modification-specific antibodies are capable of detecting proteins post-translationally modified via acetylation, methylation, cleavage, mutation and phosphorylation at certain amino acids along the target protein.

Unmodified & Total Protein Antibodies

Unmodified and total protein antibodies are capable of detecting unmodified proteins as well as total amounts of the target protein that have undergone various types of post-translational modification, respectively.

Validation Methods

Before being included in the Assay Biotech catalog, each antibody is subject to vigorous quality control testing and validation through a combination of the following applications: Western Blots (WB), Enzyme-Linked Immunosorbent Assays (ELISA), Immunohistochemistry (IHC), and Immunofluorescence (IF).

Western Blot

Western blot analysis of extracts from HeLa cells, using the RUNX2 Antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung tissue, using Phospho-ALK (Tyr1586) Antibody.

ELISA

Western blot analysis of extracts from HeLa cells, using the RUNX2 Antibody.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using the CDH23 Antibody. The picture on the right is treated with the synthesized blocking peptide.